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KMID : 0613820030130010128
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Journal of Life Science
2003 Volume.13 No. 1 p.128 ~ p.135
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Expressed Sequence Tags in Rainbow Trout (Oncorhynchus mykiss) Kidney and Microarray Analysis in Young and Old Kidney
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Kim Soon-Hak
Shin Yong-Kook
Bang In-Chul
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Abstract
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102 ESTs (Expressed Sequence Tags) were obtained by sequencing clones from a library of rainbow trout kidney cDNAs. Of the sequences generated, 55.8% of the ESTs were represented by 37 known genes. The 45 clones of unknown gene products potentially represent 40 novel genes. The genes involved in structural function (14.5%) and transcription/translation (11.6%) account for the major gene expression activities in the kidney. Microarray experiment was conducted to compare gene expression of the unique ESTs in young and adult rainbow trout kidneys. While mitochondrion, cytochrome b, rho G, spastin protein, and three unknown genes were down-regulated in the mature fish kidney, calponin 1, calcium binding protein, histone deacetylase 1, and an unknown gene were up-regulated in
the mature fish kidney. This research demonstrates the feasibility and power of functional genomics in rainbow trout.
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KEYWORD
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Expressed Sequence Tag, Expression, Microarray, Kidney, Rainbow Trout
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